A comparative study of the effects of hemin and bilirubin on bilateral renal ischemia reperfusion injury
NEPHRON EXPERIMENTAL NEPHROLOGY, cilt.103, sa.1, ss.1-5, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 103 Sayı: 1
- Basım Tarihi: 2006
- Doi Numarası: 10.1159/000090113
- Dergi Adı: NEPHRON EXPERIMENTAL NEPHROLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1-5
- Anahtar Kelimeler: bilirubin, hemin, heme oxygenase, ischemia-reperfusion injury, kidney, ISCHEMIA/REPERFUSION INJURY, OXIDATIVE STRESS, CARBON-MONOXIDE, OXYGENASE-1, INDUCTION, ANTIOXIDANT, INHIBITION, ENDOTOXIN
- Gazi Üniversitesi Adresli: Evet
Özet
Background/Aims: The aim of this study was to determine the effects of hemin, a heme oxygenase-1 inducer, and bilirubin on renal ischemia-reperfusion (I-R) injury. Methods: 40 Wistar-Albino rats were allocated into six groups as follows: sham (S), bilirubin (B), hemin (H), ischemia/ reperfusion (IR), IR + bilirubin (IRB) and IR + hemin (IRH). Conjugated bilirubin (20 mg center dot kg(-1) i. v.) was given to rats in groups B and IRB, and hemin (50 mg center dot kg (-1) i. p.) was given to rats in groups H and IRH just prior to reperfusion. Renal I-R was achieved by occluding the renal arteries bilaterally for 50 min. Following 6 h of reperfusion, blood was drawn to study BUN, creatinine and bilirubin, and tissue samples were harvested to determine the renal malonyldialdehyde and heme oxygenase-1 levels, and for histopathologic grading. Results: BUN, creatinine and malonyldialdehyde levels in group IRH were similar to controls whereas the results of groups IR and IRB were signifi cantly higher (p < 0.01). There was a grade 2 damage in all I-R groups. Conclusion: This study showed the preventive effect of hemin on renal ischemia reperfusion injury. Administration of exogenous bilirubin did not prevent the I-R injury. (C) 2006 S. Karger AG, Basel.