Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film


BAYRAMOĞLU G., Altintas B., Arica M. Y.

BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol.34, no.2, pp.127-134, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.1007/s00449-010-0453-3
  • Journal Name: BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.127-134
  • Keywords: Conductive polymer, Polyaniline, Enzyme immobilization, Adsorption, Uricase, CANDIDA-RUGOSA LIPASE, GLUCOSE-OXIDASE, COVALENT IMMOBILIZATION, CATALYTIC-PROPERTIES, POLYMER, MEMBRANES, BEADS, ACID, ADSORPTION, ENZYME
  • Gazi University Affiliated: Yes

Abstract

Polyacrylonitrile film (PAN) surfaces were modified with chemical polymerization of conductive polyaniline (PANI) in the presence of potassium dichromate as an oxidizing agent. The conductive films were used for immobilization of uricase. The surface resistance of the conductive film in this work was found to be 0.97 k Omega/cm. The maximum amount of immobilized enzyme on conductive film containing 2.4% PANI was about 216 mu g/cm(2). The optimum pH for free and immobilized enzymes was observed at 7.0 and 7.5, respectively. The K (m) values for free and immobilized uricase were found to be 94 and 138 mu M, respectively. V (max) values were calculated as 1.87 and 1.63 U/mg protein for the free and immobilized enzymes, respectively. Immobilized uricase exhibited similar to 68% of its original activity even after 2 months of storage at 4 A degrees C while the free enzyme lost its initial activity within 4 weeks.