Separation/preconcentration of Zn(II), Cu(II), and Cd(II) by Saccharomyces carlsbergensis immobilized on silica gel 60 in various samples


Baytak S., Kenduzler E., Turker A. R.

SEPARATION SCIENCE AND TECHNOLOGY, vol.41, no.15, pp.3449-3465, 2006 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 15
  • Publication Date: 2006
  • Doi Number: 10.1080/01496390600915098
  • Title of Journal : SEPARATION SCIENCE AND TECHNOLOGY
  • Page Numbers: pp.3449-3465

Abstract

This study presents a solid phase extraction procedure based on column biosorption of Zn(II), Cu(II), and Cd(II) ions on Saccharomyces carlsbergensis immobilized on silica gel 60. The analytes were determined by flame atomic absorption spectrometry (FAAS). The optimum conditions for the quantitative recovery of the analytes, including pH, amount of solid-phase, eluent type and flow rate of sample solution were examined. The effect of interfering ions on the recovery of the analytes was also investigated. Under the optimum conditions, recoveries of Zn(II), Cu(II), and Cd(II) were 99 +/- 2%, 98 +/- 2%, and 100 +/- 2% at 95% confidence level, respectively for spiked water samples. The analytical detection limits for Zn(II), Cu(II), and Cd(II) were 1.14, 1.66, and 1.48 ng mL(-1), respectively. The validation of the method was checked by the analysis of standard reference material (Tea leaves GBW-07605) and spiked water, samples. The proposed method was applied for the determination of analytes in green onion, parsley, dam water, lake water, and tap water samples. The analytes has been determined in real samples with relative error lower than 8% and relative standard deviation lower than 10%.