The effects of bumetanide on human umbilical artery contractions

Dayioglu E., Buharalioglu K., Saracoglu F., AKAR F.

REPRODUCTIVE SCIENCES, cilt.14, sa.3, ss.246-252, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1177/1933719107300871
  • Dergi Adı: REPRODUCTIVE SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.246-252
  • Anahtar Kelimeler: bumetanide, human umbilical artery, serotonin, histamine, VASCULAR SMOOTH-MUSCLE, NA+-K+-2CL(-) COTRANSPORTER, PHOSPHOINOSITIDE HYDROLYSIS, DEPENDENT VASORELAXATION, THROMBOXANE A(2), NITRIC-OXIDE, CL, VESSELS, CONTRIBUTE, HISTAMINE
  • Gazi Üniversitesi Adresli: Evet

Özet

The authors investigate the effect of bumetanide, an inhibitor of NKCC1 and a loop diuretic, on the tone of human umbilical artery (HUA). Rings of HUA (n=35) from vaginal deliveries were suspended for isometric tension recordings in organ baths. Cumulative concentration-response curves to serotonin, histamine, and KCl were performed in the absence (control) or in the presence of bumetanide. The relaxant effect of bumetanide was also evaluated in serotonin- and histmaine-induced contractions. Bumetanide inhibited HUA tone in serotonin- and histamine-induced contractions with significant changes in the potency (pD(2)) and maximum contractile response (E-max) values. However, only pD(2) values for KCl-induced contraction significantly changed in the presence of bumetanide. Bumetanide caused concentration-dependent and sustained relaxations in serotonin-induced contraction; however, there was refractoriness in histamine-induced contraction. These findings raise the possibility that NKCC1 may play a role in the regulation of the umbilical artery tone.