Individual susceptibility has a major impact on strong association between oxidative stress, defence systems and Parkinson's disease

Karahalil B., Miser Salihoğlu E., Elkama A., Orhan G., Saygın E., Akaydin Yardim S.

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, cilt.130, sa.1, ss.158-170, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 130 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1111/bcpt.13659
  • Dergi Adı: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.158-170
  • Anahtar Kelimeler: antioxidant enzymes, DNA repair, gene polymorphism, oxidative stress, Parkinson's disease, REPAIR GENE POLYMORPHISMS, DNA-REPAIR, PLASMA HOMOCYSTEINE, ROS, VITAMIN-B12, BIOMARKERS, INHIBITORS, OXIDASE, FOLATE, DAMAGE
  • Gazi Üniversitesi Adresli: Evet

Özet

Oxidative stress plays an important role in the degeneration of dopaminergic neurons, which causes Parkinson's disease (PD). Oxidative stress products, antioxidant and their balance have important roles in the development of oxidative stress-based PD. The impact of reactive oxygen species (ROS) and defence systems can be altered by genetic polymorphisms, and thus the risk of PD may also be affected. We aimed to investigate the possible association of individual susceptibility with the development of oxidative stress-based PD. For this purpose, we measured serum levels of folic acid, homocysteine, Vitamin B6 and B12 that play roles in folate-dependent one-carbon pathway, oxidant or antioxidant enzymes (NADPH oxidase, MnSOD, GPX), 8-OHdG and repair enzymes (OGG1, XRCC1 and MTH1) by ELISA, and analysed related gene polymorphisms by PCR-RFLP. XRCC1, ROS, NADPH and folic acid levels were found to be statistically higher in patients than controls. XRCC1, MnSOD and GPX activities were increased. We observed higher levels of 8-OHdG in patients with MnSOD and XRCC1 mutant genotypes and higher XRCC1 levels in patients with NOX p22 fox mutant genotypes rather than controls. We suggest that routinely clinical validation of major oxidative stress-related biomarkers will be a good approach to manage detrimental effects of PD.