Allele and genotype frequencies for D1S80 locus were studied in a sample of 362 individuals by using amplified fragment length polymorphism technique (AMP-FLP) in Turkey. There were 22 different alleles and 80 distinct genotypes observed in the Turkish population. The D1S80 locus deviated from Hardy-Weinberg equilibrium. The observed and expected heterozygosities were 0.743 and 0.807, respectively. The power discrimination was 0.94. Alleles #18 and #24 were found to be the two most common alleles in this population sample and their frequencies were 0.232 and 0.352, respectively. The allele frequencies of the Turkish population were compared with the five published population data and the two previous surveys on Turkish population by likelihood ratio test. Furthermore, the combined Turkish population data was separately compared with US African, US Caucasian, African, European and Arabic population through the same method. These comparisons revealed that the Turkish population and the combined Turkish population data were significantly different from other published population data. This study will be help to show genetic variability between different populations at D1S80 locus.