Preparation and characterization of comb type polymer coated poly(HEMA/EGDMA) microspheres containing surface-anchored sulfonic acid: Application in gamma-globulin separation

Bayramoglu G., Arica M. Y.

REACTIVE & FUNCTIONAL POLYMERS, cilt.69, sa.3, ss.189-196, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.reactfunctpolym.2008.12.017
  • Dergi Adı: REACTIVE & FUNCTIONAL POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.189-196
  • Anahtar Kelimeler: ATRP, Polymer brushes, Cation-exchange support, Protein adsorption, Human IgG, AFFINITY MEMBRANES, LYSOZYME SEPARATION, PROTEIN ADSORPTION, HIGH-CAPACITY, SPACER-ARM, PURIFICATION, BEADS, FIBER, FUNCTIONALIZATION, CHROMATOGRAPHY
  • Gazi Üniversitesi Adresli: Evet

Özet

Poly(2-hydroxyethyl methacrylate/ethylenglycol dimethacrylate), poly(HEMA/EGDMA) microspheres was prepared via suspension polymerization. After activation of the hydroxyl groups of poly(HEMA/EGDMA) by bromination, surface-initiated atom transfer radical polymerization (ATRP) of glycidylmethacrylate was conducted in dioxane/bipyridine mixture with CuBr as catalyst at 65 degrees C. The epoxy groups of the poly(glycidylmethacrylate) comb polymer were converted into sulfonic acid groups (as proton-exchange groups) with reaction of sodium sulfite. Synthesized microspheres were characterized by swelling studies, FT-IR spectroscopy, scanning electron microscopy (SEM) and elemental analysis. The microspheres were used as ion-exchange support for adsorption and purification of human gamma-globulin (IgG). The maximum gamma-globulin adsorption on the ion-exchange adsorbents was observed at between pH 5.0 and 6.0. The IgG adsorption onto the poly(HEMA/EGDMA) microspheres was negligible. The maximum amount of adsorbed gamma-globulin was found to be 230.1 mg/g microspheres. The ion-exchange adsorbents allowed one-step separation of IgG from human plasma. The gamma-globulin molecules could be repeatedly adsorbed and desorbed with this ion-exchange support without noticeable loss in their IgG adsorption capacity. (C) 2008 Elsevier Ltd. All rights reserved,