Investigation of multifaceted wound healing effect of exopolysaccharide (EPS) produced from probiotic strain <i>Lactiplantibacillus plantarum</i> GD2 as in vitro and in ovo

Demir A., ASLIM B.

SCIENTIFIC REPORTS, cilt.15, sa.1, 2025 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1038/s41598-025-90682-0
  • Dergi Adı: SCIENTIFIC REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, MEDLINE, Directory of Open Access Journals
  • Gazi Üniversitesi Adresli: Evet

Özet

Skin wounds may threaten quality of life and cause serious complications. This study aimed to investigate the effects of lyophilized exopolysaccharide (L-EPS) obtained from the probiotic strain Lactiplantibacillus plantarum GD2 on various stages of wound healing. The results revealed that L-EPS accelerated in vitro wound healing and increased COL1A1 in L929 cells. L-EPS affected the TGF-beta 1/Smad signaling pathway by increasing the expression of the TGF-beta 1, Smad2, Smad3, and Smad4 genes. L-EPS also exerted anti-inflammatory effects by reducing the gene expression of IL-1 beta, IL-6 and iNOS in TNF-alpha-induced fibroblasts. Additionally, L-EPS demonstrated fibroproliferative effect on both healthy and TNF-alpha-induced fibroblasts. Furthermore, L-EPS was found to have a proangiogenic effect in ovo chorioallantoic membrane (CAM) model. This study presents the first-ever characterization of the multifaceted effects of L-EPS derived from the probiotic strain L. plantarum GD2 on wound healing. Our findings highlight the potential of L-EPS as effective agent for wound healing and suggest possible application in the development of wound healing biomaterials. By elucidating the mechanism of action of L-EPS in wound healing, this research may provide new perspectives for advanced treatment strategies in the field of wound care.