In this study, a device that supplies a microcontroller controlled E-test strip application was developed. It was aimed to gather two devices which are needed for E-test strip application's stages, i.e., inoculation onto agar plate and placement of E-test strip, into a device to thus reduce the cost and time required for this application. A PIC16F877A microcontroller was used in the system and it was programmed in Microcode Studio Plus Editor by using the PIC Basic Programming Language. The circuit whose design was realized was tested under circumstances in the laboratory and real medium. The designed system is in a state that can be used in hospitals as it is.