Extraction of phytosterols from melon (Cucumis melo) seeds by supercritical CO2 as a clean technology

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Ekinci M. S., GÜRÜ M.

GREEN PROCESSING AND SYNTHESIS, vol.8, no.1, pp.677-682, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1515/gps-2019-0038
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.677-682
  • Keywords: supercritical carbon dioxide extraction, melon (Cucumis melo) seeds, phytosterols, beta-sitosterol, stigmasterol, FLUID EXTRACTION, CARBON-DIOXIDE, OIL
  • Gazi University Affiliated: Yes


Extraction with supercritical carbon dioxide (SC-CO2) which is known as a clean technology was carried out to extract oil from melon (Cucumis melo) seeds. SC-CO2 extraction technique does not contaminate extracts. SC-CO2 is not a toxic and a flammable solvent. Phytosterols, natural and bioactive compounds, which is known to provide protection against various chronic diseases were examined in the seed oil by using gas chromatography mass spectrometry (GC-MS). Stigmasterol and beta-sitosterol were detected in the melon seed oil. SC-CO2 extractions were performed in a range of 30-55 degrees C, 150-240 bar, 7-15 g CO2/min, 0.4-1.7 mm (mean particle size of the seeds) and 1-4 h. The optimal quantities of extracted oil, beta-sitosterol and stigmasterol were 36.8 g/100 g seed, 304 mg/kg seed and 121 mg/kg seed, respectively, at 33 degrees C, 200 bar, 11 g CO2/min, 0.4 mm and 3 h.