A green amperometric biosensor based on Aesculus hippocastanum L.-mediated silver nanoparticles for dopamine determination
BIOCHEMICAL ENGINEERING JOURNAL, sa.237, ss.1-9, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.bej.2026.110418
- Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
- Derginin Tarandığı İndeksler: Academic Search Ultimate (EBSCO), Engineering Source (EBSCO), Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS, Chimica, Compendex, EMBASE, INSPEC
- Sayfa Sayıları: ss.1-9
- Gazi Üniversitesi Adresli: Evet
Özet
Dopamine (DA) is a neurotransmitter and hormone in the brain that plays many essential roles. DA deficiency leads to various neurological disorders, especially Parkinson's disease. Therefore, it is necessary to determine DA even at low concentrations. Biosensors are bioanalytical devices that detect analytes in complex matrices. The working electrodes used in biosensor design should be modified to achieve higher selectivity. In this study, waste Aesculus hippocastanum L. was used to synthesize silver nanoparticles (AH-AgNPs) via biological synthesis. The AH-AgNPs were characterized and used to design a novel biosensor for the determination of DA. The DA was detected by reduction of the dopamine-o-quinone formed during the enzymatic reaction at −0.15 V vs Ag/AgCl. The effects of optimal operating conditions, such as temperature, pH, and substrate concentration, were investigated. In addition, the factors affecting the biosensor's performance, including the amount of AH-AgNPs, reusability, shelf life, and interference effects, were investigated. Finally, DA was quantified in a synthetic blood sample with high sensitivity and selectivity using an eco-friendly approach.