Akademik Veri Yönetim Sistemi
Toxicology Letters, cilt.314, sa.1, ss.149, 2019 (SCI-Expanded)
Effects of electrospun nanofiber curcumin on bisphenol A
exposed Caco-2 cells
*Y. Turgut1, B. Yurdakok-Dikmen1, R. Uyar1, M. Birer2, F. Acarturk2,
A. Filazi1
1 Ankara University, Faculty of Veterinary Medicine /
Department of Pharmacology and Toxicology, Ankara, Turkey;
2 Gazi University, Faculty of Pharmacy/ Department
of Pharmaceutical Technology, Ankara, Turkey
Purpose: Curcumin is the major polyphenolic compound of cur-
cuminoids, extracted from Curcuma longa L. (turmeric). Curcumin
gained increasing interest for its anti-inflammatory, anti-diabetic,
anti-carcinogenic and anti-rheumatic properties with good tolerabil-
ity and safety. However, several problems prevent marketing of cur-
cumin as a drug such as the poor aqueous solubility, intense staining
color, and extremely low oral bioavailability. In order to enhance the
solubility, curcumin loaded polyvinylpyrrolidone (PVP) K90 nanofib-
ers were prepared using electrospinning method and physicochem-
ical properties of nanofibers were characterized. Bisphenol A (BPA),
the major endocrine disruptor chemical, which stimulate estrogen
receptors at very low concentrations, induce estrogen related carcino-
genesis inducing proliferation in colon. Therefore, the aim of this study
was to determine the effects of electrospun nanofiber curcumin on
Bisphenol A treated human colorectal adenocarcinoma cells (Caco-2)
in vitro.
Methods: Electrospinning solution; consisted of PVP 12% and cur-
cumin (10mg) was prepared in ethanol. The mixture was stirred for
2 h at room temperature to obtain homogeneous solution and used
for electrospinning. Caco-2 cells (ATCC HTB-37,USA) were seeded at
80% confluence; where curcumin nanofiber at concentration of 2.7,
6.4 and 12,8 μg/ml were coincubated with BPA at 2nM-2μM. Follow-
ing 24h coexposure, MTT assay along with standard trypan blue
technique by JuLI Br Counting starter kit (NanoEnTek Inc, Seoul, South
Korea) were used.
Results: BPA induced proliferation in the cells at 8 nM. Viability of
the cells compared to untreated control against curcumin nanofibers
were 67,64±1,06 for 2.7 μg/ml, 55.12±1.12 for 6.4 μg/ml, 50,88±3.03
for 12.8 μg/ml; while BPA at 8 nM were 85.97±8.11. A significant dif-
ference were observed for curcumin nanofibers compared to BPA
only control (p<0.05); while between 6.4 and 12.8 μg/ml, no difference
were observed (p>0.05). The current study supports the enhanced
cytotoxic potential of curcumin nanofiber effective at 6.4 μg/ml con-
centration on Caco-2 colon cancer cells; where antiproliferative ef-
fects on cell proliferation induced by the environmental carcinogen
Bisphenol A were found in vitro.
References
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