Effects of electrospun nanofiber curcumin on bisphenol A exposed Caco-2 cells

Turgut Y., Yurdakök Dikmen B., Uyar R., Birer M., Acartürk F., Filazi A.

Toxicology Letters, vol.314, no.1, pp.149, 2019 (SCI-Expanded)

  • Publication Type: Article / Abstract
  • Volume: 314 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1016/j.toxlet.2019.09.002
  • Journal Name: Toxicology Letters
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.149
  • Gazi University Affiliated: Yes


Effects of electrospun nanofiber curcumin on bisphenol A

exposed Caco-2 cells

*Y. Turgut1, B. Yurdakok-Dikmen1, R. Uyar1, M. Birer2, F. Acarturk2,

A. Filazi1

1 Ankara University, Faculty of Veterinary Medicine /

Department of Pharmacology and Toxicology, Ankara, Turkey;

2 Gazi University, Faculty of Pharmacy/ Department

of Pharmaceutical Technology, Ankara, Turkey

Purpose: Curcumin is the major polyphenolic compound of cur-

cuminoids, extracted from Curcuma longa L. (turmeric). Curcumin

gained increasing interest for its anti-inflammatory, anti-diabetic,

anti-carcinogenic and anti-rheumatic properties with good tolerabil-

ity and safety. However, several problems prevent marketing of cur-

cumin as a drug such as the poor aqueous solubility, intense staining

color, and extremely low oral bioavailability. In order to enhance the

solubility, curcumin loaded polyvinylpyrrolidone (PVP) K90 nanofib-

ers were prepared using electrospinning method and physicochem-

ical properties of nanofibers were characterized. Bisphenol A (BPA),

the major endocrine disruptor chemical, which stimulate estrogen

receptors at very low concentrations, induce estrogen related carcino-

genesis inducing proliferation in colon. Therefore, the aim of this study

was to determine the effects of electrospun nanofiber curcumin on

Bisphenol A treated human colorectal adenocarcinoma cells (Caco-2)

in vitro.

Methods: Electrospinning solution; consisted of PVP 12% and cur-

cumin (10mg) was prepared in ethanol. The mixture was stirred for

2 h at room temperature to obtain homogeneous solution and used

for electrospinning. Caco-2 cells (ATCC HTB-37,USA) were seeded at

80% confluence; where curcumin nanofiber at concentration of 2.7,

6.4 and 12,8 μg/ml were coincubated with BPA at 2nM-2μM. Follow-

ing 24h coexposure, MTT assay along with standard trypan blue

technique by JuLI Br Counting starter kit (NanoEnTek Inc, Seoul, South

Korea) were used.

Results: BPA induced proliferation in the cells at 8 nM. Viability of

the cells compared to untreated control against curcumin nanofibers

were 67,64±1,06 for 2.7 μg/ml, 55.12±1.12 for 6.4 μg/ml, 50,88±3.03

for 12.8 μg/ml; while BPA at 8 nM were 85.97±8.11. A significant dif-

ference were observed for curcumin nanofibers compared to BPA

only control (p<0.05); while between 6.4 and 12.8 μg/ml, no difference

were observed (p>0.05). The current study supports the enhanced

cytotoxic potential of curcumin nanofiber effective at 6.4 μg/ml con-

centration on Caco-2 colon cancer cells; where antiproliferative ef-

fects on cell proliferation induced by the environmental carcinogen

Bisphenol A were found in vitro.


[1] Alcigir ME, Dogan HO, Yurdakok-Dikmen B., Dogan K., Vural SA, Yilmaz FM,

Isgoren A.(2018) An Investigation of the Effects of Curcumin on the Changes

in the Central Nervous System of the Rats Exposed to Arochlor 1254

in the Prenatal Period

[2] Ruzgar G., Birer M., Tort S., Acarturk F. Studies on Improvement

of Water-Solubility of Curcumin with Electrospun Nanofibers

[3] Yurdakok-Dikmen B., Alcigir ME., Dogan HO., Dogan K., Vural SA, Yilmaz FM.,

Isgoren A.(2018)Effects of Curcumin in PCB Exposed F98 Glioma Cells

[4] Xian-Yang Q, Tomokazu F, Linqing Y, Hiroko Z, Hiromi A, Qin Z,Jun Y, Hideko S.

Effects of bisphenol A exposure on the proliferation and senescence of normal

human mammary epithelial cells