Effects of electrospun nanofiber curcumin on bisphenol A exposed Caco-2 cells


Turgut Y., Yurdakök Dikmen B., Uyar R., Birer M. , Acartürk F. , Filazi A.

Toxicology Letters, vol.314, no.1, pp.149, 2019 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Abstract
  • Volume: 314 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1016/j.toxlet.2019.09.002
  • Title of Journal : Toxicology Letters
  • Page Numbers: pp.149

Abstract

Effects of electrospun nanofiber curcumin on bisphenol A

exposed Caco-2 cells

*Y. Turgut1, B. Yurdakok-Dikmen1, R. Uyar1, M. Birer2, F. Acarturk2,

A. Filazi1

1 Ankara University, Faculty of Veterinary Medicine /

Department of Pharmacology and Toxicology, Ankara, Turkey;

2 Gazi University, Faculty of Pharmacy/ Department

of Pharmaceutical Technology, Ankara, Turkey


Purpose: Curcumin is the major polyphenolic compound of cur-

cuminoids, extracted from Curcuma longa L. (turmeric). Curcumin


gained increasing interest for its anti-inflammatory, anti-diabetic,


anti-carcinogenic and anti-rheumatic properties with good tolerabil-

ity and safety. However, several problems prevent marketing of cur-

cumin as a drug such as the poor aqueous solubility, intense staining


color, and extremely low oral bioavailability. In order to enhance the


solubility, curcumin loaded polyvinylpyrrolidone (PVP) K90 nanofib-

ers were prepared using electrospinning method and physicochem-

ical properties of nanofibers were characterized. Bisphenol A (BPA),


the major endocrine disruptor chemical, which stimulate estrogen


receptors at very low concentrations, induce estrogen related carcino-

genesis inducing proliferation in colon. Therefore, the aim of this study


was to determine the effects of electrospun nanofiber curcumin on

Bisphenol A treated human colorectal adenocarcinoma cells (Caco-2)

in vitro.


Methods: Electrospinning solution; consisted of PVP 12% and cur-

cumin (10mg) was prepared in ethanol. The mixture was stirred for


2 h at room temperature to obtain homogeneous solution and used

for electrospinning. Caco-2 cells (ATCC HTB-37,USA) were seeded at

80% confluence; where curcumin nanofiber at concentration of 2.7,


6.4 and 12,8 μg/ml were coincubated with BPA at 2nM-2μM. Follow-

ing 24h coexposure, MTT assay along with standard trypan blue


technique by JuLI Br Counting starter kit (NanoEnTek Inc, Seoul, South

Korea) were used.

Results: BPA induced proliferation in the cells at 8 nM. Viability of

the cells compared to untreated control against curcumin nanofibers

were 67,64±1,06 for 2.7 μg/ml, 55.12±1.12 for 6.4 μg/ml, 50,88±3.03


for 12.8 μg/ml; while BPA at 8 nM were 85.97±8.11. A significant dif-

ference were observed for curcumin nanofibers compared to BPA


only control (p<0.05); while between 6.4 and 12.8 μg/ml, no difference

were observed (p>0.05). The current study supports the enhanced


cytotoxic potential of curcumin nanofiber effective at 6.4 μg/ml con-

centration on Caco-2 colon cancer cells; where antiproliferative ef-

fects on cell proliferation induced by the environmental carcinogen


Bisphenol A were found in vitro.

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