3200 MHZ RADYOFREKANS ALANIN MCF-7 VE L929 HÜCRE HATLARINA ETKİSİ

Aim:The use of technological devices emitting radio-frequency fields (RFF) is increasing day by day. Nowadays, low-frequency RFF has begun to be used in cancer treatment as a non- invasive method. However, the time-dependent effects of high-frequency RFF on healthy and cancer cells are not yet clear. This study aims to examine the time-dependent effects of 3200MHz RFF on human breast cancer (MCF-7) and healthy mouse fibroblast(L929) cell lines.

Methods:MCF-7 and L929 cells were cultured with DMEM medium. Cells were grown at 37°C and 5%CO2 (ThermoForma II CO2 Incubator,USA). Confluent cells were removed with trypsin solution and cell counting was performed. For cell viability testing, cells were seeded into 96-well plates (15x103cells). RFF exposures were applied at 29.55dBm power levels. MCF-7 and L929 cell lines were exposed to 3200 MHz RFF for 4 hours. Cell viability levels were determined by MTT analysis immediately after application and 2 hours later in cell lines. GraphPad-9 Macos package program was used in the analyses. The Kruskal-Wallis H test was used to determine differences between groups. A value of p<0.0001 was considered significant.

Results:Immediately after 4 hours(%12) of 3200 MHz RFF application and 2 hours(%15)after the RFF exposures, the cell viability level decreased significantly in both MCF-7 cell lines, While the L929 cell line, a significant increase in cell viability levels was observed immediately after application(27%)and 2 hours later(16%) (p<0.0001)

Conclusions:The results of this study suggest that 3200 MHz RFF exposure has a significant effect on the viability levels of cancer cells and that 3200 MHz RFF can create the effect that low-frequency fields currently used as a non-invasive method have with a significant temperature increase, without a temperature increase. It is also effective in increasing healthy fibroblast cells, suggesting that it can be used as an alternative method to wound healing and cell renewal treatments.

KEYWORDS: Radiofrequency Field, MCF-7, L929, MTT.