Differential pulse polarographic determination of selenium(IV) in whole blood using the catalytic hydrogen wave

Inam R., Ekmekci G., Somer G.

TALANTA, cilt.51, sa.4, ss.825-830, 2000 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 4
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1016/s0039-9140(99)00347-1
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.825-830
  • Gazi Üniversitesi Adresli: Evet

Özet

The selenium content in blood was determined using the hydrogen catalytic peak. This peak at -1.1 V was obtained in the presence of selenium and molybdenum at pH values of 1-4 in different buffers. For the determination of selenium, the Mo(VI) concentration has to be similar to 100-200 times higher than the selenium present. The linear domain range of selenium is 1 x 10(-6)-5 x 10(-9) M. The interference of zinc is eliminated by the addition of EDTA at pH 3.5 acetate buffer. The method was applied to 1.0 mi of digested blood, and 620 +/- 44 mu g l(-1) Se and 7.15 mg l(-1) Zn could be determined with a 90% (n = 6) confidence interval. (C) 1999 Elsevier Science B.V. All rights reserved.