With the help of TCM empirical framework in induction and expression of knowledge, this paper discusses the evolution characteristics of TCM syndrome differentiation knowledge in the information environment, so as to promote the information construction of TCM to meet the dynamic requirements of TCM practice. The empirical framework of TCM was properly transformed so that the knowledge of TCM can be expressed in the information system. The self-organization evolution model of TCM syndrome differentiation knowledge was established. Taking the "fever" symptom and its related symptoms in Treatise on Febrile Diseases as the basic information source and the running results of the evolutionary model are obtained by computer simulation method. The simulation shows that the evolution of syndrome differentiation knowledge is based on similarity of information, and the low conflict information filling is the main way to obtain the results of diversity evolution. These findings are echo with the characteristics of cumulative and progressive development of TCM and the cautious principle of TCM syndrome differentiation.