Effects of beta-Glucan on Colon Anastomotic Healing in Rats Given Preoperative Irradiation


Seker A., Deger K. C., Bostanci E. B., Ozer I., Dalgic T., Bilgihan A., ...More

JOURNAL OF INVESTIGATIVE SURGERY, vol.27, no.3, pp.155-162, 2014 (SCI-Expanded) identifier identifier identifier

Abstract

Background: Radiation therapy is an essential therapeutic modality in the management of a wide variety of tumors. We aimed to investigate the short-term effects of pelvic irradiation on the healing of colon anastomoses and to determine the potential protective effects of beta-glucan in this situation. Material and Methods: Sixty Wistar albino rats were randomized into three experimental groups: a control group (n = 20), an irradiation (IR) group (n = 20), and an irradiation + beta-glucan (IR + beta-glucan) group (n = 20). Only segmental colonic resection and anastomosis were performed on the control group. The IR group underwent the same surgical procedure as the control group 5 days after pelvic irradiation. In the IR+ beta-glucan group, the same procedure was applied as in the IR group after beta-glucan administration. The groups were subdivided into subgroups according to the date of euthanasia (third [n = 10] or seventh [n = 10] postoperative [PO] day), and anastomotic colonic segments were resected to evaluate bursting pressures and biochemical and histopathological parameters. Results: Bursting pressure values were significantly lower in the IR group (p < .001). Malondialdehyde (MDA) levels were significantly higher in the IR group, whereas beta-glucan significantly decreased MDA levels on the third PO day (p < .001). Granulation tissue formation scores were significantly lower in the IR+ beta-glucan group compared with the control group and the IR group (p < .001). Conclusions: The results of this study indicate that irradiation has negative effects on the early healing of colon anastomoses. The administration of beta-glucan ameliorates these unfavorable effects by altering bursting pressures and biochemical parameters.