The protective effect of pentoxifylline and L-arginine on 60 minutes normothermic liver ischemia and reperfusion injury in rats RATLARDA 60 DAKIKA KARACIGER ISKEMI REPERFUZYON HASARINDA PENTOKSIFILIN VE L-ARGININ'IN KORUYUCU ETKISI
Turkish Journal of Surgery, cilt.12, sa.4, ss.249-254, 1996 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 4
- Basım Tarihi: 1996
- Dergi Adı: Turkish Journal of Surgery
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.249-254
- Anahtar Kelimeler: Ischemia reperfusion injury, L-arginine, Liver ischemia, Nitric oxide, Pentoxifylline
- Gazi Üniversitesi Adresli: Evet
Özet
We investigated the protective effects of pentoxifylline and L-arginine, a precursor of nitric oxide, on 60 minutes normothermic liver ischemia and reperfusion injury in rats. Hepatic artery, vena ports and common bile duct were clamped at the level of hilus for 60 minutes with atraumatic microvascular clamp end followed by 2 hours of reperfusion. Liver tissue samples were removed for malondialdehyde (MDA) and reduced glutathione (GSH) determinations, and histopathological examination. Four groups of 10 rats each were constituted: Group 1: (Sham operated: No IR Injury), Group 2: (Control: Saline treatment), Group 3: (Pentoxifylline treatment) and Group 4: (L-arginine treatment). There were significant increases in tissue MDA levels, more cellular damage and significant decrease in tissue GSH levels in control group, when compared with other groups (p<0.05). Our results demonstrated that pentoxifylline and L-arginine are able to prefect the liver from normothermic ischemia and reperfusion injury.