EVALUATION OF MICRONUCLEI IN EXFOLIATED UROTHELIAL CELLS AND URINARY THIOETHER EXCRETION OF SMOKERS


BURGAZ S., ISCAN A., BUYUKBINGOL Z., BOZKURT A., KARAKAYA A.

MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS, vol.335, no.2, pp.163-169, 1995 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 335 Issue: 2
  • Publication Date: 1995
  • Doi Number: 10.1016/0165-1161(95)90052-7
  • Journal Name: MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.163-169
  • Keywords: MICRONUCLEI, UROTHELIAL CELLS, URINARY THIOETHER, SMOKER, HUMAN BUCCAL MUCOSA, BLADDER-CANCER, CIGARETTE SMOKERS, HUMAN EXPOSURE, BITUMEN FUMES, SMOKING, CARCINOGENESIS, MUTAGENICITY, TOBACCO, MARKER
  • Gazi University Affiliated: Yes

Abstract

Mutagens are present in large quantities in the urine of cigarette smokers, thus, their urothelial cells may represent a possible target for absorbed and excreted mutagens. Our aim is to validate the micronucleus (MN) test in exfoliated urothelial cells obtained from urine samples of cigarette smokers. The urinary thioether (UT) test is also carried out on the same individuals in order to find out whether there is any correlation between these two end-points. The mean (+/-SE) MN frequency and UT determination is 1.93 (+/-0.11)% and 9.71 (+/-1.61) mmol SH/mol creatinine for 23 smokers, and 0.66 (+/-0.05)% and 4.20 (+/-0.56) mmol SH/mol creatinine for 20 nonsmokers. Our results show a higher frequency of micronucleated cells (p < 0.001) and higher excretion of UTs (p < 0.05) in smokers as compared to nonsmokers. Concentrations of UTs and MN frequencies increased with tobacco consumption. The MN frequencies showed only a marginal increase, not significant (p > 0.05), after passive smoking compared to nonsmoking values. There was no significant correlation between MN frequencies and UTs, either in smokers (r = 0.164, p > 0.05) or in nonsmokers (r = -0.018, p > 0.05). Our data demonstrate tobacco-induced chromosome damage in bladder tissue consistent with increased risk of cancer at this site among smokers.