Novel acridines (4a - 4l) were synthesized by reaction of tetraketones (3a, 3b) and aromatic amines. The structures of compounds were characterized by FT-IR, H-1 NMR, mass spectroscopy, and elemental analysis. Furthermore, all the synthesized compounds were tested in vitro for their antimicrobial and antifungal activity. The results were compared with conventional reference antibiotics. Many of the acridine compounds considerably reacted against Esherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, and Staphylococcus aureus. Particularly, compound 4k showed more pronounced activity than reference antibiotics against Salmonella enteritidis. All the compounds showed moderate activities against Candida albicans and Candida glabrata.