Akademik Veri Yönetim Sistemi
Microchimica Acta, cilt.191, ss.1-13, 2024 (Hakemli Dergi)
A new, sensitive, and cost-efective lab-on-paper-based immunosensor was designed based on electrochemical impedance
spectroscopy (EIS) for the detection of exosomes. EIS was selected as the determination method since there was a surface
blockage in electron transfer by binding the exosomes to the transducer. Briefy, the carbon working electrode (WE) on the
paper electrode (PE) was modifed with gold particles (AuPs@PE) and then conjugated with anti-CD9 (Anti-CD9/AuPs@
PE) for the detection of exosomes. Variables involved in the biosensor design were optimized with the univariate mode.
The developed method presents the limit of detection of 8.7× 102
exosomes mL−1, which is lower than that of many other
available methods under the best conditions. The biosensor was also tested with urine samples from cancer patients with
high recoveries. Due to this a unique, low-cost, biodegradable technology is presented that can directly measure exosomes
without labeling them for early cancer or metastasis detection