Designing and application of a new medical instrument sterilization system using reactive oxygen species

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Ulkutas H. O., Özçelik B., Güler İ.

REVIEW OF SCIENTIFIC INSTRUMENTS, vol.92, no.11, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 92 Issue: 11
  • Publication Date: 2021
  • Doi Number: 10.1063/5.0065104
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, INSPEC, MEDLINE, MLA - Modern Language Association Database
  • Gazi University Affiliated: Yes


The aim of this study is to design and test a new medical sterilization system as an alternative to the techniques used in the sterilization of medical instruments. The designed system, which uses a new oxygen molecule allotrope (NOMA) in the reactive oxygen species, is developed as an alternative to the sterilization systems using other gases. The test was conducted on 12 different materials, each having a surface of 2 cm(2), sterilized under 120 & DEG;C at 1 atm pressure for 20 min in the NUVE-OT 4060 sterilizer, and all surfaces were contaminated with a biological indicator Geobacillus stearothermophilus cultured in an incubator at 37 & DEG;C. Test samples in sterile Petri dishes were placed in a desiccator, and a sample was taken at 30-, 45-, and 60-min test periods and were placed on an agar medium and put in a Memmert incubator IN75 at 37 & DEG;C; in the controls conducted following 16-18 h of incubation period, no bacterial growth was observed in the newly designed system tested with gram positive bacilli; moreover, it was observed that the new system was 100% effective in sterilizing the microbes as no growth was observed on the samples. Within the scope of this study, a faster, low-cost, low-temperature, residue-free, and human and environmental friendly system was developed and tested for sterilization of medical devices compared to existing sterilization methods using NOMA.