In this study, it is purposed to determine of reactive aggregate ratio of the perlite aggregate for given up the highest length change. For this purpose, properties of relate to alkali-silica reaction (ASR) of the perlite aggregate determined with non-reactive limestone aggregate. For the determination of ASR, according to ASTM C 1260 accelerated mortar bar method, for microstructure; Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX) analysis were carried out on the samples which are perlite, limestone and used both of them. Mixtures were prepared by weight at 0, 10, 20, 30, 40, 50 and 100% ratios with usage of perlite aggregate that obtained from Ankara Cubuk region instead of limestone aggregate that obtained from Ankara Hasanoglan region. 21 pieces of mortar bars were produced for 7 different mixtures. The length change values of the samples were measured on the 3, 7, 10, 14, 21 and 28-day according to the accelerated mortar bar method. Morphology and chemical composition of highest length changed samples were determined with SEM and EDX. Increase of fracture density and Ca ratio was observed in parallel with the increase of length change. According to average of 14 days length change, 40% perlite aggregated samples underwent highest length change and these samples were determined as pesimum reactive aggregate ratio of the perlite aggregate.