The Effect of Taurine on the Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures

PAŞAOĞLU Ö. T., Turkozkan N., ARK M., Polat B., Agilli M., Yaman H.

INFLAMMATION, vol.37, no.5, pp.1439-1443, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.1007/s10753-014-9868-3
  • Journal Name: INFLAMMATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1439-1443
  • Keywords: nitric oxide, ADMA, homocysteine, HUVEC, taurine, NITRIC-OXIDE SYNTHASE, ENDOTHELIAL DYSFUNCTION, PLASMA HOMOCYSTEINE, CELLS, PATHWAY, DIMETHYLARGININE
  • Gazi University Affiliated: Yes

Abstract

The aim of our study was to investigate the effect of taurine on the relationship between nitric oxide (NO), asymmetric dimethylarginine (ADMA) and homocysteine (Hcy) in endotoxin-induced human umblical vein endothelial cell (HUVEC) cultures. For this reason, four groups were formed (n = 12). Control group consists of HUVEC cultures without any treatment. Lipopolysaccharide (LPS) and LPS + taurine groups were treated with 10 mu g/mL endotoxin, 5 mu g/mL taurine and endotoxin + taurine (same doses), respectively. Nitrite/nitrate (NOx), ADMA and Hcy levels were measured. There was a significant increase of NOx, ADMA and Hcy in endotoxemia (p < 0.05). Taurine treatment elevated NOx levels significantly (p < 0.01) in taurine and LPS + taurine group compared to control group, while it reduced NOx levels compared to LPS group. In contrast, taurine decreased ADMA levels to the control level both in taurine and taurine + LPS group compared to LPS. Hcy levels increased significantly compared to taurine group (p < 0.05) and did not change compared to LPS group. Taurine was effective on ADMA-NO relationship whereas no beneficial effect was observed in Hcy levels (p < 0.05).