Effects of Early Interventions on Behavioral Development, Prefrontal Cortex and Hippocampus NR2B Subunit Level of Adult Wistar Rats

Küçükkarapınar M. , Candansayar S.

Noropsikiyatri Arsivi-Archives Of Neuropsychiatry, cilt.11, sa.1, ss.1-10, 2020 (SCI Expanded İndekslerine Giren Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Konu: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.29399/npa.24943
  • Dergi Adı: Noropsikiyatri Arsivi-Archives Of Neuropsychiatry
  • Sayfa Sayıları: ss.1-10


Objective: Interventions performed in the early period of life are associated with cognitive and behavioral changes in adulthood. The effects of interventions such as exposure to an early stressful life event or environmental enrichment on cognitive and behavioral development are the areas studied. The aim of this study is to develop a new intervention method, to investigate the effects of early interventions on social interaction, memory anxiety levels and NR2B (N methyl D aspartate Receptor Subunit 2B) levels in prefrontal and hippocampus in adulthood. The hypothesis of the study is that exposure to the ambivalent mother will negatively affect the behavioral performance and neurodevelopment of the rats in adulthood as much as being separated for one hour from the mother, and that it will also cause changes in NR2B levels in the prefrontal cortex and hippocampus. Method: In the study, the Wistar rats were divided into four groups as control group (12), group that remained 15 minutes apart from mother (mild stress) (12), group that remained 60 minutes apart from mother (severe stress) (14) and ambivalent mother group (13). In adulthood, the social interaction test, raised plus maze and new object recognition test performances of rats were evaluated. ELISA method was used to evaluate the effects of the interventions on the prefrontal cortex and hippocampus NMDA R2B levels. Results: The important findings of the study were that in the new object recognition test, separation from the mother did not affect the recognition memory regardless of gender, while the short-term recognition memory of the females of the ambivalent mothers group was good and the long-term memory performance of the mild stress group males were better. In addition, in the social interaction test, the males of the ambivalent group and the severe stress group showed more aggressive behavior. It was determined that the prefrontal cortex NR2B level was higher in mild stress and ambivalent mother group compared to the severe stress group, and NR2B level was increased in all intervention groups compared to the control group. Conclusion: In contrast to the hypothesis, the results of this study support that the behaviors and neurodevelopments of the ambivalent mother group rats are not more adversely affected than the severe stress group rats, and that the model created may be an environmental enrichment model rather than an early stressful life event exposure. Keywords: wistar rats, behavioral manipulations, NR2B NMDA receptor, aggression,