Determination of pK(a) Values of Some Antipsychotic Drugs by HPLC-Correlations with the Kamlet and Taft Solvatochromic Parameters and HPLC Analysis in Dosage Forms


ŞANLI S., AKMEŞE B., ALTUN Y.

JOURNAL OF AOAC INTERNATIONAL, vol.96, no.1, pp.60-66, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 96 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.5740/jaoacint.11-494
  • Title of Journal : JOURNAL OF AOAC INTERNATIONAL
  • Page Numbers: pp.60-66

Abstract

In this study, ionization constant (pK(a)) values were determined by using the dependence of the retention factor on the pH of the mobile phase for four ionizable drugs, namely, risperidone (RI), clozapine (CL), olanzapine (OL), and sertindole (SE). The effect of the mobile phase composition on the PKa was studied by measuring the pK(a), at different acetonitrile water mixtures in an HPLC-UV method. To explain the variation of the pKa values obtained over the whole composition range studied, the quasi-lattice quasi-chemical theory of preferential solvation was applied. The pK(a) values of drugs were correlated with the Kamlet and Taft solvatochromic parameters. Kamlet and Taft's general equation was reduced to two terms by using combined factor analysis and target factor analysis in these mixtures: the independent term and the hydrogen-bond donating ability alpha. The HPLC-UV method was successfully applied for the determination of RI, OL, and SE in pharmaceutical dosage forms. CL was chosen as an internal standard. Additionally, the repeatability, reproducibility, selectivity, precision, and accuracy of the method in all media were investigated and calculated.