A new fast reversed-phase ultra-performance liquid chromatographic approach for quantitative analysis of pantoprazole in tablets


Dermis S., Büker E., Ertekin Z. C. , Dinç E.

Asian Journal of Chemistry, vol.27, no.12, pp.4675-4678, 2015 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 12
  • Publication Date: 2015
  • Doi Number: 10.14233/ajchem.2015.19332
  • Journal Name: Asian Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.4675-4678
  • Keywords: Pantoprazole, Reversed phase ultra-performance liquid chromatography
  • Gazi University Affiliated: No

Abstract

A new fast reversed-phase ultra-performance liquid chromatographic (RP-UPLC) approach was developed for the determination of pantoprazole in tablets. Chromatographic separation and quantitation was achieved isocratically on a Waters UPLC BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm) using a mobile phase containing the mixture of methanol and 0.05 M H3PO4 (40:60, v/v) at a flow rate of 0.25 mL/min with a PDA detection at 288 nm. Ondansetron was used as an internal standard. The retention time of pantoprazole and ondansetron was reported as 1.69 and 1.15 min, respectively. Calibration curve for the analyzed drug was obtained by using the linear concentration range of 2.0-40.0 μg/mL with eight different concentration levels. The developed RP-UPLC method was validated using recovery studies, inter-day, intra-day experiments and standard addition assays. It was concluded that the proposed and validated RP-UPLC approach can be used for the quality control and routine analysis of pantoprazole in commercial tablets.