INDIAN JOURNAL OF TRADITIONAL KNOWLEDGE, cilt.22, sa.1, ss.211-219, 2023 (SCI-Expanded)
Bioactivities of a number of medicinal plants; Alkanna tinctoria (L.) Tausch, Alnus glutinosa (L.) Gaertn., Calamintha
nepeta Willk. and C. nepeta, Centaurea iberica Trevir. ex Spreng., Citrus paradisi Macfad., C. paradisi, Citrus sinensis (L.)
Osbeck, Colutea cilicica Boiss. & Balansa, Cotinus coggygria Scop., Cuscuta arvensis Beyr. ex Engelm., Equisetum
palustre L., Lapsana communis L., Laurus nobilis L., Olea europea L., Plantago major L., Rhus coriaria L, Salvia
verticillata L., Sambucus ebulus L., Sedum acre L, Thymus capitatus (L.) Hoffmanns. & Link, T. capitatus, Thymbra
spicata L., T. spicata (n: 20), which are used for the prevention and treatment of diverse diseases, were investigated.
The antimicrobial activities of extracts were evaluated using broth microdilution assay. The cytotoxicities of extracts were
investigated on HeLa cell line by MTT assay. Statistical analysis was performed using GraphPad Prism (5.0). The effects of
the extracts, which have the highest antimicrobial activity, on the Escherichia coli and Staphylococcus aureus DNA gyrase
gene expression were determined by using quantitative reverse transcription polymerase chain reaction (qRT-PCR).
The MICs (µg/ml) of extracts were determined as 32-64, 2-128, 8-128,1-128, 4-128 against Gram-positive, Gram-negative
bacteria, yeasts, dermatophytes, and Mycobacterium spp., respectively. No cytotoxicity has been observed in plant extracts
tested. DNA gyrase activity was determined for T. capitatus-SFE (128 µg/mL) and L. nobilis-Hx (128 µg/mL) extracts
according to the inhibition of DNA gyrase gene expression. Overall, T. capitatus-SFE and L. nobilis-Hx are good candidates
for further antimicrobial studies.