Cocoa powder is obtained from Theobroma cacao L. (Malvaceae), which is often used for flavoring in food industry. It is also well-known to be rich in polyphenols, which are beneficial for human health including neuroprotection. As cholinergic hypothesis proposed for pathology of Alzheimer’s disease (AD), cholinergic deficit has been reported due to hydrolysis of acetylcholine by acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). In this study, inhibitory effect of ethanol extracts of the cocoa powders with different brands purchased from several supermarkets in Ankara (Türkiye) against AChE and BChE closely related to AD was evaluated. Moreover, the impact of the same extracts on the elimination of free radicals, which affect the formation and progression of AD, was studied. The antioxidant effects of the samples were determined by 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), and metal-chelating activity assays. Total phenolic and total flavonoid contents in the extracts were also determined by Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. Among our samples, the sample coded as RY12 had a high AChE inhibition activity by 56.46 ± 2.12% (inhibition% ± standard deviation, S.D.) at a final concentration of 200 μg/mL. On the other hand, the sample coded as RY7 had a strong BChE inhibition activity by 41.29 ± 0.09% with a final concentration of 200 μg/mL. DPPH radical scavenging activity, FRAP, and metal- chelating activity experiments revealed that the ethanol extracts had modest antioxidant activity. The total flavonoid content of the samples could not be determined since the concentration of quercetin in all samples except of RY5 was less than 0.016 mg. According to the results of total phenol quantification calculated on the basis of gallic acid in the extracts, where each sample was determined to contain moderate phenol content. The results of the study showed that the ethanol extracts of cocoa powders purchased from the markets and used by the public in daily life have a moderate level of cholinestarase inhibition and have antioxidant activity at some extent that can be evaluated. Further studies are in progress in our laboratory to determine which substances are responsible for antioxidant and antialzheimer activities.