An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline-polyvinylsulfonate-potassium ferricyanide film

ARSLAN F., Beskan U.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.42, no.4, pp.284-288, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 4
  • Publication Date: 2014
  • Doi Number: 10.3109/21691401.2013.812650
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.284-288
  • Keywords: amperometric biosensor, ferricyanide, glucose, polyaniline, polyvinylsulphonate, POLYPYRROLE FILM, ELECTRODE, FERROCENE, MEDIATOR, SENSORS
  • Gazi University Affiliated: Yes


In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate-potassium ferricyanide (Pani-Pvs-Fc) films has been accomplished via the entrapment technique. Potassium ferricyanide was used as the mediator. Determination of glucose was carried out by the oxidation of potassium ferrocyanide at 0.3 V vs. Ag/AgCl. The effects of pH and temperature were investigated, and the optimum pH value was found to be 7.5. The storage stability and the operational stability of the enzyme electrode were also studied.