An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline-polyvinylsulfonate-potassium ferricyanide film

ARSLAN F., Beskan U.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.42, sa.4, ss.284-288, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3109/21691401.2013.812650
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.284-288
  • Anahtar Kelimeler: amperometric biosensor, ferricyanide, glucose, polyaniline, polyvinylsulphonate, POLYPYRROLE FILM, ELECTRODE, FERROCENE, MEDIATOR, SENSORS
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate-potassium ferricyanide (Pani-Pvs-Fc) films has been accomplished via the entrapment technique. Potassium ferricyanide was used as the mediator. Determination of glucose was carried out by the oxidation of potassium ferrocyanide at 0.3 V vs. Ag/AgCl. The effects of pH and temperature were investigated, and the optimum pH value was found to be 7.5. The storage stability and the operational stability of the enzyme electrode were also studied.