Genetic Variations in the Hypoxia-Inducible Factor-1 alpha Gene and Lung Cancer


KONAÇ E. , DOĞAN İ. , ÖNEN H. İ. , YURDAKUL A. S. , ÖZTÜRK C. , Varol A., ...More

EXPERIMENTAL BIOLOGY AND MEDICINE, vol.234, no.9, pp.1109-1116, 2009 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 234 Issue: 9
  • Publication Date: 2009
  • Doi Number: 10.3181/0902-rm-49
  • Title of Journal : EXPERIMENTAL BIOLOGY AND MEDICINE
  • Page Numbers: pp.1109-1116

Abstract

Hypoxia-inducible factor-1 (HIF-1), an important genetic component of angiogenesis, becomes stable as a response to tumor hypoxia and facilitate!; tumor survival. The polymorphisms of the HIF-1 alpha gene may cause changes in the activity of this protein, which serves as a transcription factor for many genes in tumorigenesis. In this study, we have investigated the relationship between seven HIF-1 alpha polymorphisms [C > T substitution in intron 8 (rs10873142), T4181 (rs41508050) in exon 10, P564P (rs41492849), L580L (rs34005929), P582S (rs11549465), A588T (rs11549467) in exon 12 and dinucleotide GT repeats in intron 13 (rs10645014)] among lung cancer patients in the Turkish population. Genomic DNA was isolated from 141 lung cancer cases and 156 controls and subjected to PCR for amplification. Genotyping was carried out with RFLP and DNA sequencing methods. There was no significant difference between the lung cancer cases and controls in terms of the distribution of genotyping frequencies of seven HIF-1 alpha polymorphisms (P > 0.05). No significant reationship was found between the C > T substitution in intron E; and P582S haplotypes and development of lung cancer. In addition, there were no significant associations between the genotypes and clinopathological characteristics of the cases examined. These findings show that polymorphisms in the HIF-1 alpha gene do not confer susceptibility to lung cancer. Exp Biol Med 234:1109-1116, 2009