Purpose: The aim of this study was to find the most effective ovarian hyperstimulation protocol for patients over 35 years old. Materials and Methods: This is a retrospective study of 390 first IVF cycles of patients older than 35 years, that had serum follicle stimulating hormone < 10 IU/L, and that had no co-existing endocrine disorders. Long (n=181), antagonist (n=71), and micro-dose flare-up (n=138) protocols were evaluated. Results: Clinical pregnancy and live birth rates were highest in long protocol group and lowest in micro-dose protocol group. The difference between long and micro-dose protocol groups was statistically significant (p < 0.05). In multivariate logistic regression analysis, picked-up oocyte count (p = 0.005), endometrium thickness at hCG day (p = 0.006), age (p = 0.006), and antral follicle numbers (p = 0.013) were found to be predictive for obtaining clinical pregnancy. Treatment protocols were not found to be predictive for obtaining clinical pregnancy (p > 0.05). Conclusion: Treatment protocols were not found to be predictive for obtaining clinical pregnancy. Patient's age, antral follicle number, endometrial thickness at hCG day, and picked-up oocyte counts directly effect the pregnancy rates. Long protocol affects these factors positively can be preferred in younger patients with higher antral follicle numbers.