Antioxidant and Anticholinesterase Assets and Liquid Chromatography-Mass Spectrometry Preface of Various Fresh-Water and Marine Macroalgae


Murat K., Ilkay O. , Mahmud A., Senol F. S. , Tahir A. , Bilge S.

PHARMACOGNOSY MAGAZINE, vol.5, no.20, pp.291-297, 2009 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 20
  • Publication Date: 2009
  • Doi Number: 10.4103/0973-1296.58147
  • Title of Journal : PHARMACOGNOSY MAGAZINE
  • Page Numbers: pp.291-297
  • Keywords: Algae, Aquatic plants, Antioxidant activity, Acetylcholinesterase inhibition, Total phenol content, LC-DAD-MS, ALGAE, SEAWEEDS, CAROTENOIDS, GLYCOSIDES, SPIROGYRA

Abstract

The ethanol extracts from the fresh-water algae; Chara hispida L., Cladophora glomerata (Dilw.) Kutz, C. fracta (Dilw.) Kutz, Spirogyra gratiana Transeau, Mougeotia sp. (C.A. Agardh), Vaucheria sessilis (Vauch.) De Candolle, Geminella mutabilis (Breb.) Wille, the fresh-water plants; Ranunculus rionii Lagger and Ceratophyllum demersum L., as well as the marine algae; Sciniaia furcellata (Turn.) J. Agardh, Dictyota dichotoma (Huds.) Lam., Padina vickersiae Hoyt, Halopteris scoparia (L.) Sauvagau, and the sea grass; Posidonia oceanica (L.) Dell. were assessed in vitro for their antioxidant and anti-acetylcholinesterase (AChE) activities. Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity test at 0.125, 0.25, 0.5, 1.0, and 2.0 mg ml(-1) and ferric-reducing antioxidant power assay (FRAP) at 0.5, 1.0, and 2.0 mg ml(-1) concentrations. Total phenolic contents of the extracts were determined using Folin-Ciocalteau's reagent. The extracts were further analyzed qualitatively by LC-DAD-MS. S. gratiana had the best antioxidant activity, followed by R. rionii. LC-DAD-MS showed rich gallic acid and its ethyl ester contents in S. gratiana, while prosperous vitamin C content has been detected in P. oceanica for the first time in this study. S. gratiana possessed the highest inhibition (42.5 +/- 2.28%) at 2.0 mg ml-1 against AChE.