The purpose of this study was to investigate the effect of tooth agenesis on dentofacial structures according to the location of the absent teeth. A total of 74 subjects were classified to three main groups and four subgroups according to the location of the absent teeth. Thirteen subjects without tooth agenesis were selected as the control group. All subjects were evaluated cephalometrically. As a result it can be concluded that tooth agenesis has little effect on dentofacial structures. Although there were statistically significant differences between groups, generally mean values were within the normal range.