Investıgatıon of Nicotinamide Phosphorıbosyltransferase (Nampt), Interleukin-6 (Il-6) And Vaspin Serum Protein Levels In Type 2 Diabetes Mellitus


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Çelik S. P. , Parıltı D. N. , Yunusov E., Açık L., Yalçın M. M. , Yetkin İ.

11th International Conference "Acb-2022": Achievements And Challenges in Biology, Baku, Azerbaijan, 13 - 14 October 2022, pp.138-139

  • Publication Type: Conference Paper / Summary Text
  • City: Baku
  • Country: Azerbaijan
  • Page Numbers: pp.138-139
  • Gazi University Affiliated: Yes

Abstract

In type 2 diabetes (T2D) and obesity, imbalances are seen in the production of adipokines released from adipose tissue. Adipose tissue synthesizes several adipokines, such as adiponectin, leptin, resistin, vaspin, and the recently discovered nicotinamide phosphoribosyltransferase (NAMPT). It has been suggested that these molecules may explain the relationship with obesity, insulin resistance, beta-cell dysfunction and cardiovascular diseases. Increased insulin secretion in eNAMPT (extracellular NAMPT) β cells provides protection from apoptosis and induction of proliferation. The interaction between the insulinlike effects of NAMPT and its binding to the insulin receptor suggests that it may play a role in the pathogenesis of diabetes. Many studies show that changes in NAMPT levels constitute a risk factor for type 2 diabetes and there is a correlation between them [Grolla et al., 2016; Garten et al., 2015]. Interleukin-6 is a proinflammatory cytokine. It is involved in regulating the immune response, acute phase response and inflammation [Akbari et al., 2018]. IL-6 triggers insulin resistance by acting on the mTOR, STAT-3 and SOCS-3 pathways in the liver. Chronic low-grade inflammation is a risk factor for the development of insulin resistance and type 2 diabetes, which is manifested by high circulating IL-6 levels in T2D patients. High IL-6 concentrations suppress insulin secretion, and increased IL-6 may cause loss of function of pancreatic islet cells [Qu et al., 2014]. Vaspin (visceral adipose tissue-derived serpin) is an adipocytokine belonging to the serpin protease inhibitor family with insulin-sensitizing and anti-inflammatory effects. It is thought to be involved in the negative regulation of gluconeogenesis and the regulation of the lipid metabolic process. It has also been observed that induction of vaspin mRNA expression by human adipose tissue constitutes a compensatory mechanism against obesity, glucose tolerance and insulin sensitivity [Liu et al., 2017]. For this reason, it is aimed to study NAMPT, IL-6 and Vaspin protein levels, which contribute to the development of insulin resistance and diabetic complications in T2D, on groups at different stages of the disease and to understand the effects of differences in protein levels on the disease. The study was carried out with 3 different patient groups, aged between 18-70 years, with a known diagnosis of T2D for 10 years, with macrovascular and microvascular complications, without complications, and healthy control group, who applied to Gazi University Medical Faculty Hospital Endocrinology and Metabolism Department. 40 people were included in each group. Blood samples were taken from volunteers and serum part was separated and Enzyme-Linked ImmunoSorbent Assay (ELISA) study was performed with serum samples. Protein levels of study groups (Without complication, Microvascular complication, Macrovascular complication, Healthy (Control)) were determined according to Enzyme-Linked ImmunoSorbent Assay (ELISA) results (ng/µl). NAMPT levels in the groups: 18.693, 18.490, 16.563, 20.588; IL-6 levels: 24.844, 27.325, 30.687, 24.352; Vaspin levels were determined as 27.204, 30.737, 33.552 and 28.346. According to the obtained data, serum levels of NAMPT were higher in the control group than in the patient groups. Among the patient groups, protein levels decreased further as complications increased. When IL-6 and Vaspin serum levels were evaluated, it was determined that protein levels, which were low in the control group, increased as complications increased. Low levels of NAMPT observed in advanced stages of diabetes suggest that NAMPT has a protective effect in diabetes, while increased levels of IL-6 and Vaspin suggest that these proteins play a role in the progression and pathogenesis of the disease.