Akademik Veri Yönetim Sistemi
33rd International Symposium on Pharmaceutical and Biomedical Analysis PBA 2023, Ankara, Türkiye, 02 Temmuz 2023, ss.229
Ways of prevention of fire have been an issue and subject
of various studies for a very long time. Flame Retardants (FRs)
can be defined as compounds which can be used to reduce
f
lammability and stop fires from starting and spreading (Yao et
al.,2021). FRs have a wide range of usage including furniture,
electrical devices, construction materials and components in
transportation vehicles (Shaw et al.,2014). However some FRs
have a critical disadvantage which is their quality to leach from the
treated materials (Yao et al.,2021). Exposure to these chemicals in
every period of human life has become inevitable in our current
living conditions. Accumulating scientific evidence shows that
FRs are important members of endocrine disruptors and the
increasing amount of toxicity information about the exposure due
to the use of these substances has become a general concern.
It is seen that most of the studies examined the presence
of FRs by liquid chromatography-tandem mass spectrometer
(LC-MS/MS) in wastewater, air, dust and materials in daily use.
Today LC-MS/MS analysis which is one of the analysis methods
used for the determination of FRs exposure in human, requires
very complex studies, simple and fast analysis studies are not
commonly found in literature. The biological materials that are
used in these limited number of studies include urine, whole
blood, breast milk (Bastiaensen et al., 2018; Giroud et al.,2021;
Kakimoto et al.,2008). In our study we tried to determine FRs
exposure in urine samples as biological material and analysis was
conducted with LC-MS/MS. When studies investigating the
urinary exposures in different societies are evaluated, it is seen
that most of these biomonitoring studies include an extraction
phase which is mainly solid phase extraction (Bastiaensen et
al., 2018). However in our study analysis was conducted with a
dilution process instead of solid phase extraction. In this study
our effort was to develop an accurate, fast and sensitive LC-MS/
MS method in order to determine various organophosphate
f
lame retardants(OPFRs) concentrations in human urine and
validate this specific method. Within the scope of this study
levels of Tris (1,3-dichloro isopropyl) phosphate (TDCPP),
Tris (2-chloroethyl) phosphate (TCEP), Triphenyl phosphate
(TPHP), tris(2-butoxyethyl)phosphate (TBOEP) were
quantitatively determined by LC / ESI-MS / MS in negative
mode, using Reverse phase C-18 column. Confirmed method
was successfully conducted on analysis of spot urine samples
of randomly selected healthy children. An effort was made to
determine very low levels of TDCPP, TCEP, TPHP and TBOEP
in human urine by this validated LC-ESI MS / MS method. As a
result LOD and LOQ values of analyzed FRs were set.
Keywords: LC-MS/MS, OPFRs, flame retardants