Anticholinesterase, antioxidant, analgesic and anti-inflammatory activity assessment of Xeranthemum annuum L. and isolation of two cyanogenic compounds


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ERDOĞAN ORHAN İ. , Gulyurdu F., AKKOL E. , ŞENOL DENİZ F. S. , Anul S. A. , Tatli I. I.

PHARMACEUTICAL BIOLOGY, vol.54, no.11, pp.2643-2651, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 11
  • Publication Date: 2016
  • Doi Number: 10.1080/13880209.2016.1177092
  • Title of Journal : PHARMACEUTICAL BIOLOGY
  • Page Numbers: pp.2643-2651

Abstract

Context:Xeranthemum annuum L. (Asteraceae) (XA) is an ornamental and medicinal species with limited bioactivity and phytochemical data.Objective: Identification of anticholinesterase, antioxidant, anti-inflammatory and analgesic effects of the flower and root-stem (R-S) extracts of XA.Materials and methods: Anticholinesterase (at 100gmL(-1)) and antioxidant (at 1000gmL(-1)) effects of various extracts were evaluated via microtiter assays, while anti-inflammatory and analgesic effects of the R-S extracts were tested using carrageenan-induced hind paw oedema (100 and 200mg kg(-1)) and p-benzoquinone (PBQ) writhing models (200mg kg(-1)) in male Swiss albino mice. The R-S ethanol extract of XA was subjected to isolation studies using conventional chromatographic methods.Results: Most of the extracts showed inhibition over 85% against butyrylcholinesterase and no inhibition towards acetylcholinesterase. The flower chloroform and the R-S ethyl acetate extracts were most effective (97.850.94% and 96.89 +/- 1.09%, respectively). The R-S ethanol extract displayed a remarkable scavenging activity against DPPH (77.33 +/- 1.99%) and in FRAP assay, while the hexane extract of the R-S parts possessed the highest metal-chelating capacity (72.79 +/- 0.33%). The chloroform extract of the R-S caused a significant analgesic effect (24.4%) in PBQ writhing model. No anti-inflammatory effect was observed. Isolation of zierin and zierin xyloside, which were inactive in anticholinesterase assays, was achieved from the R-S ethanol extract.Discussion and conclusion: This is the first report of anticholinesterase, antioxidant, analgesic and anti-inflammatory activities and isolation of zierin and zierin xyloside from XA. Therefore, XA seems to contain antioxidant and BChE-inhibiting compounds.