In this study, different thickness varnish layers were applied by using cellulosic, polyurethane, acrylic and water based varnishes on pine (Pinus sylvestris L.), fir (Abies Bornmulleriana Mattf), Eastern beech (Fagus orientalis L.), and oak (Quercus petraea L.) wood surfaces. Effects of wood species and different thickness varnish layers on adhesion strength of varnish films were determined with a pull-off test according to the ASTM-D 4541. Results of the study indicated that the effect of wood species and varnish type are significant in adhesion, while the effect of layer thickness is not significant. In addition, it was deduced that the strength of adhesion is higher in hardwoods than softwoods. The highest strength was measured with acrylic and polyurethane varnishes. Tests also showed that failures mostly occur on the interface of first coat (filling coat) and the wood surface. Thus, it can be concluded that the final coat (top coat) does not have any effect on the strength of adhesion.