In this study, total number of 160 canned tuna fish samples were investigated the presence and levels of Bisphenol A (BPA) in four brands (A, B, C, D) of canned tuna fish samples which were collected from Ankara region, Turkey. Additionally, the salt amount, pH value and drain weight level for each sample were determined. Quantitative analysis of BPA in samples was carried out by solid phase extraction method followed by High Performance Liquid Chromatography (HPLC) with fluorescence detection. The mean levels of BPA in the analysed canned fish samples were determined as 0.45 +/- 0.09, 0.31 +/- 0.09, 0.45 +/- 0.09 and 2.33 +/- 0.09 mg kg(-1) for A, B, C, D brands, respectively. According to the findings of this study, it was determined that the mean BPA values of the samples except for the values obtained from D brand were below the Turkish Food Codex (TFC) values (0.6 mg kg(-1)). Furthermore, the pH values and salt amounts were in accordance with Turkish Standart Instute (TSI) values for all brands. However, mean of drain weight found in all brands were lower than the TSI value. In conclusion, some samples obtained during the period of the study are determined to have high levels of BPA. Therefore, the steps in all food processing should be monitored for preventing the BPA contamination.