Apolipoprotein E (Apo E) is important in plasma lipid metabolism and has three major isoforms which are encoded by three allelic forms, epsilon 2, epsilon 3, epsilon 4. To explore the involment of apolipoprotein E gene in alcoholism, Apo E genotypes were evaluated after the digestion with Cfol restriction enzyme in 52 type 11 alcoholics and 120 healthy controls. The frequencies of ApoE alleles in alcoholics were 13% in epsilon 2, 81% in epsilon 3 and 6% in epsilon 4. The corresponding frequencies of the three alleses in control subjects were 7%, 89% and 4%, respectively. There were no significant differences in the distribution of Apo E genotypes and alleles between the alcoholics and the controls (p > 0.05).