Metabolism-induced oxidative stress is a mediator of glucose toxicity in HT22 neuronal cells

Rackova L., Snirc V., Jung T., Stefek M., KARASU Ç., Grune T.

FREE RADICAL RESEARCH, cilt.43, sa.9, ss.876-886, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 9
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1080/10715760903104374
  • Dergi Adı: FREE RADICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.876-886
  • Anahtar Kelimeler: Metabolic oxidative stress, hyperglycaemia, diabetes, neuronal cells, proteasome, protein oxidation, INDUCED DIABETIC-RATS, PYRIDOINDOLE ANTIOXIDANT STOBADINE, STREPTOZOTOCIN-TREATED MICE, LIPID-PEROXIDATION, OXIDIZED PROTEINS, INTRACELLULAR-DISTRIBUTION, CARDIAC MYOCYTES, NITRIC-OXIDE, BETA-CELLS, HIPPOCAMPUS
  • Gazi Üniversitesi Adresli: Evet

Özet

Oxidative stress has been widely considered as a key player in the adverse effects of hyperglycaemia to various tissues, including neuronal cells. This study examined the participation of oxidative stress in injurious effects of high glucose on HT22 cells along with the activity of proteasome, a proteolytic system responsible for degradation of oxidized proteins. Although 10-fold glucose concentration caused non-significant viability changes, a significant reduction of cell proliferation was found. Moreover, the cell morphology was also altered. These changes were followed by an enhancement of intracellular ROS generation, however without any significant boost of the carbonyl group concentration in proteins. Correspondingly, only a slight decline in the 20S proteasome activity was found in high-glucose-treated cells. On the other hand, substances affecting glucose metabolism or antioxidants partially preserved the oxidative stress in high glucose treated cells. In summary, these results highlight the role of metabolic oxidative stress in hyperglycaemia affecting neurons.