Effects of diabetes and elevated glucose on nitrergic relaxations in the isolated duodenum of the rat


Utkan T., Yildiz F., Utkan N. Z. , Gacar N., GÖÇMEZ S. S. , ULAK G., ...More

ACTA DIABETOLOGICA, vol.46, no.4, pp.295-301, 2009 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 4
  • Publication Date: 2009
  • Doi Number: 10.1007/s00592-008-0086-z
  • Title of Journal : ACTA DIABETOLOGICA
  • Page Numbers: pp.295-301
  • Keywords: Diabetes mellitus, Nitric oxide, Duodenum, Hyperglycemia, Rat, OXIDE-MEDIATED NEUROTRANSMISSION, ENDOTHELIUM-DEPENDENT RELAXATION, ALDOSE REDUCTASE INHIBITOR, NITRIC-OXIDE, ANOCOCCYGEUS MUSCLE, ELECTRICAL-STIMULATION, GLYCOSYLATION PRODUCTS, AUTONOMIC NEUROPATHY, LONGITUDINAL MUSCLE, AORTA

Abstract

Nitrergic relaxations of the isolated duodenum, induced by streptozotocin, were investigated in the experimental 8-week diabetes rat model. The effects of elevated glucose were also examined in the incubated duodenal muscles (in Krebs-Henseleit solution containing 44 mM glucose for 6 h) taken from nondiabetic rats. The relaxations induced by electrical field stimulation (EFS) and nicotine were significantly reduced in diabetic rats compared with control rats. Incubating of duodenal tissues in medium containing elevated glucose revealed significantly impaired relaxations to EFS and nicotine compared to responses obtained after normal glucose incubation. However, the relaxant responses to sodium nitroprusside and papaverine were similar in all groups. Incubating in hyperosmolar solutions containing sucrose, the relaxant responses were not affected. In conclusion, impairment of NO-mediated relaxations in diabetes may be related to hyperglycemia. The alterations caused by elevated glucose are not due to a hyperosmotic effect because the same concentration of sucrose had no effect on the relaxations.