The present study was conducted in order to determine the effect of a training programme on behavioural self-regulation skills of preschool children. For this purpose, the research was carried out as an experimental study based on pre-test-post-test model with a control group. The study group consisted of 54 children. In this study, Head-Toes-Knees-Shoulders was used to determine the behavioural self-regulation skills and Child Behavior Rating Scale was used to find out teacher opinions. Since the sample group did not show normal distribution, non-parametric tests were used in the analysis of the data. In order to determine the effectiveness of the programme applied to experimental group, Mann-Whitney-U and Wilcoxon marked rank tests were used in the comparison of the pre-test and post-test scores. As a result of the study, significant differences were found between the experimental and control groups in favour of the experimental group children in terms of the post-test scores.