The Effect of Ultrasonic Liposuction Energy Levels on Fat Graft Viability

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Genç İ. G., Fındıkçıoğlu K., Sadioğlu A., Erdal A. I., Özkoçer S. E., Elmas Ç.

AESTHETIC PLASTIC SURGERY, cilt.46, sa.5, ss.2509-2516, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00266-022-02824-8
  • Dergi Adı: AESTHETIC PLASTIC SURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.2509-2516
  • Anahtar Kelimeler: VASER, Lipectomy, Graft survival, Perilipin-1, ADIPOCYTES, LIPOPLASTY, RETENTION, HARVEST, DEATH, CELLS, MODEL, VIVO
  • Gazi Üniversitesi Adresli: Evet

Özet

Abstract
Background The use of fat obtained from ultrasound-assisted
liposuction is popular. However, no study has considered
the effect of different energy levels on fat grafts.
Objectives We hypothesized that different ultrasonic
energy levels could change the fat graft viability.
Methods Both flanks of 15 CD1 nude mice (30 experimental
areas) were used, with experimental areas randomly
distributed into five groups. Using different energy settings,
fat grafts were obtained from a patient’s abdominoplasty
material and applied to the mouse flank regions. Device
settings were intermittent mode with 50% vibration
amplitude in group 1, continuous mode with 50% vibration
amplitude in group 2, intermittent mode with 90% vibration
amplitude in group 3, and continuous mode with 90%
vibration amplitude in group 4. The control group was
grafted with fat obtained via the conventional method.
After 6 weeks, all mice were sacrificed, and fat grafts were
excised. Sections were stained with hematoxylin–eosin,
Masson’s trichrome, and anti-perilipin A antibody.
Results The perilipin A immunostaining result was lowest
in group 4, indicating the lowest viable cell count (p \
0.01). There was no significant difference between groups
for the other parameters (p[0.05).
Conclusion High ultrasonic energy may affect fat graft
survival. If fat injection is planned, avoiding high energy
settings (our recommendation is not to exceed 16 Watts.)
should be considered. We also recommend increasing the
vibration amplitude rather than switching from intermittent
to continuous mode in body parts that are relatively resistant
to liposuction.
No Level Assigned This journal requires that authors
assign a level of evidence to each submission to which
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excludes Review Articles, Book Reviews, and manuscripts
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Keywords VASER  Lipectomy  Graft survival 
Perilipin-1