The effect of Rho kinase inhibitor Y-27632 on endotoxemia-induced intestinal apoptosis in infant rats


ÖZDEMİR D., CİLAKER MIÇILI S., TUĞYAN K., Dagdelen M. K. , DERİNÖZ GÜLERYÜZ O., Guneli E.

JOURNAL OF MOLECULAR HISTOLOGY, vol.43, no.1, pp.81-87, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.1007/s10735-011-9379-6
  • Journal Name: JOURNAL OF MOLECULAR HISTOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.81-87
  • Keywords: Endotoxemia, Infant rats, Intestinal apoptosis, Rho kinase inhibitor, Y-27632, OXIDATIVE STRESS, EPITHELIAL APOPTOSIS, ENDOTHELIAL-CELLS, FOCAL ADHESIONS, FAMILY GTPASES, LIVER-INJURY, MICE, MELATONIN, SURVIVAL, SEPSIS
  • Gazi University Affiliated: Yes

Abstract

The aim of this study was to evaluate the effect of Rho kinase inhibitor, Y-27632 on the intestinal apoptosis in endotoxemic infant rats. Wistar albino 15-17-day-old rat pups (n = 21) were randomized to three experimental groups: (1) controls; (2) endotoxemia (LPS); and (3) endotoxemia treated with Y-27632 (LPS + Y-27632). Endotoxemia was induced in rats by intraperitoneal (i.p) injection of lipopolysaccharide (Escherichia coli serotype 0111:B4; 10 mg/kg). Y-27632 was administered 5 mg/kg i.p at three times, just, 8 and 16 h after LPS injection. Twenty-four hours after LPS injection, intestinal apoptosis was assessed by hematoxylin and eosin staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and immunohistochemistry for active caspase-3. Endotoxemia induced extensive apoptotic injury in the intestinal tissues. The administration of Y-27632 to endotoxemic infant rats caused a marked decrease in the number of apoptotic cells in both intestinal epithelium and lamina propria. In conclusion, the inhibition of Rho kinase with Y-27632 diminished the intestinal apoptotic damage induced by endotoxemia in infant rats.