Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.6, sa.2, ss.125-134, 2009 (Scopus)
In the present study, nine extracts of Centaurea tchihatcheffii were screened for their in-vitro
antiviral, antibacterial and antifungal activities. Antibacterial and antifungal activities were evaluated
against both standard and the isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus
mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus
faecalis, Bacillus subtilis as well as Candida albicans, Candida parapsilosis by broth microdilution
method. Susceptibility testing was performed according to the Clinical and Laboratory Standards
Institute (CLSI; formerly NCCLS). Furthermore, Herpes simplex virus Type-1 (HSV-1) and
Parainfluenza-3 virus (PI-3 virus, were employed for antiviral assessment of the extracts by using
Madin-Darby Bovine Kidney and Vero cell lines. Ampicilline, gentamicin, ofloxacin, levofloxacin,
ketoconazole, fluconazole, acyclovir and oseltamivir were used as the control agents. As our
knowledge, this is the first report on these C. tchihatcheffii extracts that were evaluated for their
antimicrobial activities. According to the data obtained, all the extracts appear to have antibacterial
activity tested against standard Gram-negative and Gram-positive bacteria ranging from minimum
inhibitory concentrations of 2 to 16 fig/mL, besides they have have antibacterial activity at 32->128
jUg/mL concentrations to isolated strains. Notable activity was observed with the extracts against C
albicans and C. parapsilosis fungi at 4 fig/mL and 8 fig/mL concentrations, respectively.The data
showed that water-chloroform interpahse (U.i), chloroform (U.2), and ethyl acetate (U.3), have antiviral
activity against both DNA (HSV-1) and KNA (PI-3) viruses