Akademik Veri Yönetim Sistemi
Journal of Ethnopharmacology, cilt.357, 2026 (SCI-Expanded, Scopus)
Ethnopharmacological relevance: Achillea biebersteinii Afan. is traditionally utilized as folk medicine for a broad range of therapeutic applications, especially for promoting the maturation of abscesses, wound healing; against inflammation, and rheumatism in Türkiye. Aim of the study: The anti-inflammatory potential of A. biebersteinii was evaluated through activity-guided fractionation (AGF) targeting lipoxygenase (15-LOX) inhibition. Materials and methods: Different chromatographic techniques were used for the AGF of the ethyl acetate extract of A. biebersteinii aerial parts. The in vitro 15-LOX inhibitory activity evaluation was performed to address the anti-inflammatory activity. The structures of purified compounds from the fractions were confirmed by LC-HRMS,1H NMR, and13C NMR analyses, respectively. Results: The fractionation and isolation process culminated in the identification of three key flavonoids namely; patulitrin, axillarin, quercetagetin-7-O-β-glucopyranoside, and 4,5-dicaffeoylquinic acid, which showed statistically remarkable 15-LOX inhibitory activity with inhibition rates of 82.27 %, 96.81 %, 84.65 % and 77.47 %, respectively. Two flavonoids were isolated by using the AGF method, where quinic acid was spotted to have significant 15-LOX inhibitory activity. Conclusion: These findings support the future therapeutic potential of A. biebersteinii as a natural anti-inflammatory source.