Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk


Atakisi O., Oral H., Atakisi E., Merhan O., Pancarci S. M. , Ozcan A., ...More

RESEARCH IN VETERINARY SCIENCE, vol.89, no.1, pp.10-13, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 89 Issue: 1
  • Publication Date: 2010
  • Doi Number: 10.1016/j.rvsc.2010.01.008
  • Journal Name: RESEARCH IN VETERINARY SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.10-13
  • Keywords: Nitric oxide, Free radical, Milk, Subclinical mastitis, NECROSIS-FACTOR-ALPHA, ESCHERICHIA-COLI, OXIDATIVE STRESS, CLINICAL MASTITIS, INFLAMMATION, INJURY, PEROXYNITRITE, NEUTROPHILS, INDUCTION, INFECTION
  • Gazi University Affiliated: No

Abstract

The aim of this study was to investigate total antioxidant (TAC), and oxidant capacity (TOC) and nitric oxide (NO) levels in milk of cows with subclinical mastitis. Brown Swiss and Holstein breed cows were screened with California Mastitis Test (CMT) to determine mammary glands with subclinical mastitis. Moreover, somatic cell counts (SCC) were determined electronically in all milk samples. Mammary quarters were classified as healthy (n = 25) or subclinical mastitis (n = 35) based on CMT scores and somatic cell count (SCC: <= 200,000/ml or >200,000/ml) in milk. Nitric oxide, TOC and SCC levels were significantly higher (p <0.001, p < 0.005 and p <0.001. respectively) in milk from mammary quarters with subclinical mastitis compared to those from healthy mammary quarters. In conclusion, subclinical mastitis results in higher NO concentrations, TOC and SCC, and NO and TOC were positively correlated with SCC. Moreover, alterations in NO levels and TOC in milk could be used as an alternative diagnostic tool to screen for subclinical mastitis. (C) 2010 Elsevier Ltd. All rights reserved.