Akademik Veri Yönetim Sistemi
Ankara Üniversitesi Tıp Fakültesi Mecmuası, cilt.76, sa.1, ss.30-36, 2023 (Hakemli Dergi)
Objectives: Interleukin-33 (IL-33) is a pleiotropic cytokine from the IL-1 family. It is known to cause plasticity in the Th2 direction. The macrophages, important cells of the innate immune system, express the IL-33 receptor ST2L and exhibit M2 macrophage polarity under the influence of IL-33. Macrophages produce interferon gamma-stimulated protein 10 (IP-10), an interferon-γ-induced chemokine, and neopterin which exhibits cellular immunoreaction. The aim of this study was to investigate the effects of IL-33 on neopterin and IP-10 production in unstimulated and prestimulated macrophages with different cytokines. Materials and Methods: The J774.1 macrophage cell line was used. J774.1 macrophages were stimulated by IFN-γ (40 U/mL) + LPS (10 ng/mL) after pretreatment with IL-33 (100 ng/mL). Nitrit levels were measured by the Griess reaction. To examine the effect of IL-33 on polarized macrophages, J774.1 macrophages were stimulated with IL-4, IL-10 ve IL-13 cytokines to provide M1, M2a and M2c macrophage polarization. Neopterin and IP-10 measurements were carried out by ELISA method. Statistical analysis was performed with SPSS 18 software. Results: IL-33 was found to significantly increase neopterin production in unstimulated and plasticity-affected J774.1 cells. There was no change in IP-10 levels in all experiments. No correlation was found between neopterin and IP-10 levels. Conclusion: Our present study is the first study on the effects of IL-33 cytokine on neopterin production. IL-33 causes a significant increase in neopterin production in J774.1 cells.