Cholinesterase and tyrosinase inhibitory, and antioxidant potential of randomly selected Umbelliferous plant species and the chromatographic profile of Heracleum platytaenium Boiss. and Angelica sylvestris L. var. sylvestris


ERDOĞAN ORHAN İ. , Tosun F., Skalicka-Wozniak K.

JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, vol.81, no.4, pp.357-368, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 81 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.2298/jsc150902017o
  • Title of Journal : JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
  • Page Numbers: pp.357-368
  • Keywords: neurobiological activity, coumarins, Apiaceae, HPLC, IN-VITRO, ETHANOLIC EXTRACTS, MEDICINAL-PLANTS, NEUROTOXICITY, SCOPOLAMINE, DYSFUNCTION, COUMARINS, STRESS, MICE

Abstract

The neurobiological activity of the methanol extracts of thirteen Umbelliferae (Apiaceae) plants was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) using a high-throughput screening technique. Although the extracts displayed no to a low inhibition profile against the enzymes, the highest cholinesterase inhibition was observed with Heracleum platytaenium (32.52 +/- 3.27 % for AChE and 46.16 +/- 1.42 % for BChE) at 100 mu g mL(-1). Since neurodegeneration is linked to oxidative damage, the antioxidant potential of the extracts were examined through radical scavenging, metal-chelating capacity, and reducing power experiments and they exerted modest levels of activity varying according to the method. The extracts had a better ability to scavenge the nitric oxide radical (19.47 +/- 2.09 to 54.91 +/- 1.98 %). Since these species are known to be rich in coumarins, quantitative high-performance liquid chromatography (HPLC) analysis indicated the presence of xanthotoxin, angelicin, isopimpinellin, bergapten, and pimpinellin in Heracleum platytaenium and angelicin and imperatorin in Angelica sylvestris var. sylvestris.