Akademik Veri Yönetim Sistemi
Hacettepe Journal of Biology and Chemistry, cilt.52, sa.4, ss.273-283, 2024 (Hakemli Dergi)
Globally, there is a significant demand for novel agents capable of providing protection against both pathogenic microorga-
nisms and tumor cells. In this study, the antimicrobial, biofilm inhibitory, and anticancer effects of glycine and glycyl-glycine
were investigated. The antimicrobial effects were determined using the broth dilution method, while the biofilm inhibitory effects
were assessed through the crystal violet binding assay. Cytotoxic effects on HeLa cell and A549 cell line viability were measured
using the MTT assay. Our results indicate that, although 100 mg/mL of glycine only inhibited Staphylococcus epidermidis W17
among the three tested isolates, 400 mg/mL of glycyl-glycine inhibited both S. epidermidis W17 and Proteus mirabilis U15 strains.
Additionally, sub-MICs (concentrations below the Minimum Inhibition Concentration) of glycine inhibited biofilm formation by
more than 70% in all tested clinical isolates and exhibited significantly more biofilm inhibition against P. mirabilis U15 and S. epider-
midis W17 strains (p<0.05). A549 and HeLa cell types exhibited a significant reduction in cell viability at concentrations of 0.3 mM
and 1 mM compared to the control after treatment with glycine and glycyl-glycine (p<0.001). The results obtained in this study
will enhance the development of new strategies using amino acids and dipeptide-based synthesizable molecules, contributing to
advancements in drug development research.